The Chinese University of Hong Kong, Hong Kong Special Administrative Region
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons
Wai Wa Ray Chan *1, Wen Li *1,2, Dik Long Dennis Chau *1, Kwok-Fai Lau 1
1School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 2Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen
In this report, we describe a simple protocol for studying neurite outgrowth in embryonic rat cortical neurons by co-transfecting with EGFP and the protein of interest.
Biochemistry
Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
Wai Wa Ray Chan *1, Yu Qi Zhai *1, Kwok-Fai Lau 1
1School of Life Sciences, The Chinese University of Hong Kong
This paper aims to present a protocol for preparing recycling endosomes from mammalian cells using sucrose density gradient ultracentrifugation.
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