Univ. Lille, INSERM

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Quentin Lemaire¹, Marie Duhamel¹, Antonella Raffo-Romero¹, Michel Salzet¹, Christophe Lefebvre¹

¹U1192-Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), Univ. Lille, INSERM

The present report highlights chronological requirements for extracellular vesicle (EV) isolation from microglia or blood macrophages. Microglia-derived EVs were evaluated as regulators of the neurite outgrowth while blood macrophage-derived EVs were studied in the control of C6 glioma cell invasion in in vitro assays. The goal is to better understand these EV functions as immune mediators in specific microenvironments.