登录

Consejo Superior de Investigaciones Científicas - Universidad Autónoma de Madrid, CIBERONC

1 ARTICLES PUBLISHED IN JoVE

image

Cancer Research

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
Cristina Galindo-Pumariño 1, Alberto Herrera 2, Alberto Muñoz 3, Alfredo Carrato 1, Mercedes Herrera 4, Cristina Peña 1
1Medical Oncology Department, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), CIBERONC, 2Department of Medical Oncology, Hospital Universitario Puerta de Hierro de Majadahonda, 3Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas - Universidad Autónoma de Madrid, CIBERONC, 4Department of Oncology & Pathology, Karolinska Institutet

The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. Fibroblasts are seeded in a pre-treated culture plate and stimulated with ascorbic acid for matrix generation. Matrices are decellularized and blocked to culture relevant cells (e.g., endothelial cells).

JoVE Logo

政策

使用条款

隐私

科研

教育

关于 JoVE

版权所属 © 2024 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。