Biodynamics Research Center (BDR), RIKEN

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Ahmed Ali^1,2, Yasmine Abouleila^1,2, Arno Germond¹

¹Biodynamics Research Center (BDR), RIKEN, ²Research Center, Misr International University

This protocol presents an integrated Raman spectroscopy-mass spectrometry (MS) platform that is capable of achieving single-cell resolution. Raman spectroscopy can be used to study cellular response to drugs, while MS can be used for targeted and quantitative analysis of drug uptake and metabolism.