Heidelberg University Children's Hospital

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Leukocyte Infiltration of Cremaster Muscle in Mice Assessed by Intravital Microscopy

Simon Alexander Kranig^*1, Trim Lajqi^*1, Raphaela Tschada¹, Maylis Braun¹, Navina Kuss¹, Johannes Pöschl¹, Hannes Hudalla¹

¹Department of Neonatology, Heidelberg University Children's Hospital

Here, we show how to perform intravital microscopy on post-capillary venules of the mouse cremaster muscle. Commonly applied to different models of inflammation and sepsis, particularly those induced by chemokines and cytokines, we highlight its relevance in the study of muscolopathies involving exaggerated muscular leukocyte infiltration.