Instituto de Neurobiología, UNAM
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Daniela Avila-González 1, Guadalupe García-López 1, Néstor E. Díaz-Martínez 2, Héctor Flores-Herrera 3, Anayansi Molina-Hernández 1, Wendy Portillo 4, Néstor F. Díaz 1
1Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología, 2Biotecnología Médica y Farmacéutica CONACYT, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), 3Departamento de Inmunobioquímica, Instituto Nacional de Perinatología, 4Instituto de Neurobiología, UNAM
This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.
Neuroscience
Culture of Neurospheres Derived from the Neurogenic Niches in Adult Prairie Voles
Daniela Ávila-González 1, Larry J. Young 2, Francisco Camacho 1, Raúl G. Paredes 1,3, Néstor F. Díaz 4, Wendy Portillo 1
1Departamento de Neurobiología Conductual y Cognitiva, Instituto de Neurobiología, Universidad Nacional Autónoma de México, 2Silvio O. Conte Center for Oxytocin and Social Cognition, Center for Translational Social Neuroscience, Department of Psychiatry and Behavioral Sciences, Yerkes National Primate Research Center, Emory University, 3Escuela Nacional de Estudios Superiores Juriquilla, Universidad Nacional Autónoma de México, 4Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología
We established the conditions to culture neural progenitor cells from the subventricular zone and dentate gyrus of the adult brain of prairie voles, as a complementary in vitro study, to analyze the sex-dependent differences between neurogenic niches that could be part of functional plastic changes associated with social behaviors.
