Berlin Institute of Health

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Brain Death Induction in Mice Using Intra-Arterial Blood Pressure Monitoring and Ventilation via Tracheostomy

Paul V. Ritschl^*1,2,3, Lena Hofhansel^*2,4, Bernhard Flörchinger⁵, Rupert Oberhuber², Robert Öllinger¹, Johann Pratschke¹, Katja Kotsch⁶

¹Department of Surgery Campus Charité Mitte/Campus Virchow-Klinikum, Charité - Universitätsmedizin Berlin, ²Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, ³Charité Clinician Scientist Program, Berlin Institute of Health, ⁴Department of Psychiatry, Psychotherapy and Psychosomatics, RWTH Aachen University, ⁵Department of Cardiothoracic Surgery, University Medical Center Regensburg, ⁶Department of General, Visceral and Vascular Surgery, Charité - Universitätsmedizin Berlin

We present a murine model of brain death induction in order to evaluate the influence of its pathophysiological effects on organs as well as on consecutive grafts in the context of solid organ transplantation.

Immunology and Infection

Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat

Lavinia Curini^*1,2, Mary Roxana Christopher^*1, Herko Grubitzsch^3,4, Ulf Landmesser^1,3, Amedeo Amedei^*2,5, Alexander Lauten^*1,6, Brunilda Alushi^*1,6

¹Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), ²Department of Experimental and Clinical Medicine, University of Florence, ³Berlin Institute of Health, ⁴Department of Cardiology, German Heart Centre Berlin (DHZB), ⁵Sod of Interdisciplinary Internal Medicine, Azienda Ospedaliera Universitaria Careggi (AOUC), ⁶Department of General and Interventional Cardiology, Helios Klinikum Erfurt

In this study, we describe the process of T lymphocyte isolation from fresh samples of calcified aortic valves and the analytical steps of T cell-cloning for the characterization of the adaptive leukocyte subsets by using flow cytometry analysis.