UC Davis School of Medicine
3 ARTICLES PUBLISHED IN JoVE
Biology
DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
Elva Diaz 1, Gustavo A. Barisone 1
1Department of Pharmacology, University of California, Davis
We demonstrate the use of DNA microarrays for expression profiling of the nervous system. We describe RNA quality control, sample labeling, and array hybridization and scanning.
Medicine
Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
Angel Alvarez 1, Gustavo A. Barisone 1, Elva Diaz 1
1Department of Pharmacology, University of California, Davis
The Focus Formation Assay provides a straightforward method to assess the transforming potential of a candidate oncogene.
Biochemistry
Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
David J. Speca 1, Elva Diaz 1
1Department of Pharmacology, UC Davis School of Medicine
Palmitoylation entails the incorporation of a 16-carbon palmitate moiety to cysteine residues of target proteins in a reversible manner. Here, we describe a biochemical approach, the acyl-PEGyl exchange gel shift (APEGS) assay, to investigate the palmitoylation state of any protein of interest in mouse brain lysates.
