Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging
Maria Lucia Pigazzini *1,2, Christian Gallrein *1, Manuel Iburg *1, Gabriele Kaminski Schierle 3, Janine Kirstein 1,4
1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin, 2NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, 3Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology, University of Cambridge, 4Cell Biology, University of Bremen
Fluorescence lifetime imaging monitors, quantifies and distinguishes the aggregation tendencies of proteins in living, aging, and stressed C. elegans disease models.
Biology
In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2
Maria Lucia Pigazzini 1,2, Janine Kirstein 1,3
1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund, 2NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, 3Department of Biology and Chemistry, University of Bremen
Presented here is a protocol to monitor degradation of the protein huntingtin fused to the photoconvertible fluorophore Dendra2.
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