Ospedale Pediatrico Bambino Gesù, IRCCS
2 ARTICLES PUBLISHED IN JoVE
Medicine
Three-Dimensional (3D) Tumor Spheroid Invasion Assay
Maria Vinci 1,2, Carol Box 2, Suzanne A. Eccles 2
1Division of Molecular Pathology, The Institute of Cancer Research, 2Division of Cancer Therapeutics, The Institute of Cancer Research
Invasion of surrounding normal tissues is a defining characteristic of malignant tumors. We provide here a simple, semi-automated micro-plate assay of invasion into a natural 3D biomatrix that has been exemplified with a number of models of advanced human cancers.
Biology
Measuring the Confluence of iPSCs Using an Automated Imaging System
Valentina Magliocca 1,2, Maria Vinci 3, Tiziana Persichini 2, Franco Locatelli 3, Marco Tartaglia 1, Claudia Compagnucci 1
1Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS, 2Department of Science, University Roma Tre, 3Department of Onco-hematology, Gene and Cell Therapy, Ospedale Pediatrico Bambino Gesù, IRCCS
The goal of the protocol is to compare different extracellular matrix (ECM) coating conditions to assess how differential coating affects the growth rate of induced pluripotent stem cells (iPSCs). In particular, we aim to set up conditions to obtain optimal growth of iPSC cultures.
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