Enforced Activation of Enhancer RNAs In Situ through the dCas9 Synergistic Activation Mediator SystemZian Liao *1,2, Joo-Hyung Lee *1, Joanna Krakowiak 1, Ruoyu Wang 1,2, Wenbo Li 1,2
1Department of Biochemistry and Molecular Biology, McGovern Medical School, University of Texas Health Science Center, 2Graduate School of Biomedical Sciences, University of Texas MD Anderson Cancer Center and UTHealth
Enhancer RNAs (eRNAs) are non-coding RNAs produced from active enhancers. An optimal approach to study eRNA functions is to manipulate their levels in the native chromatin regions. Here we introduce a robust system for eRNA studies by using CRISPR-dCas9-fused transcriptional activators to induce the expression of eRNAs of interest.
