Université Grenoble Alpes, CEA, CNRS, IBS
2 ARTICLES PUBLISHED IN JoVE
Chemistry
Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
Sofia Jaho 1, Niels Junius 1,3, Franck Borel 1, Yoann Sallaz-Damaz 1, Jean-Baptiste Salmon 2, Monika Budayova-Spano 1
1Université Grenoble Alpes, CEA, CNRS, IBS, 2CNRS, Solvay LOF, UMR 5258, Université Bordeaux, 3ELVESYS SAS
This paper details the fabrication protocol of microfluidic chips developed for on-chip protein crystallization with the dialysis method and in situ X-ray diffraction experiments. The microfabrication process makes it possible to integrate a semipermeable regenerated cellulose dialysis membrane with any molecular weight cut-off, between two layers of the chip.
Biology
Optimization of Crystal Growth for Neutron Macromolecular Crystallography
Elham Vahdatahar 1, Niels Junius 1,2, Monika Budayova - Spano 1
1CEA, CNRS, IBS, Université Grenoble Alpes, 2ELVESYS SAS
Structural studies of biomacromolecules by crystallography require high-quality crystals. Here we demonstrate a protocol that can be used by OptiCrys (a fully automated instrument developed in our lab) and/or microdialysis buttons for growing large high-quality crystals based on knowledge of the crystallization phase diagram.
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