An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular AngiogenesisYohei Tomita 1, Zhuo Shao 2, Bertan Cakir 1, Yumi Kotoda 1, Zhongjie Fu 1,3, Lois E.H. Smith 1
1Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, 2Department of Clinical and Metabolic Genetics, Hospital for Sick Children, University of Toronto, 3Manton Center for Orphan Disease, Harvard Medical School, Boston Children's Hospital
This protocol presents choroid sprouting assay, an ex vivo model of microvascular proliferation. This assay can be used to assess pathways involved in proliferating choroidal micro vessels and assess drug treatments using wild type and genetically modified mouse tissue.
