Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic MarkersMowei Zhou 1, Shadan H. Abdali 1, David Dilworth 2, Lifeng Liu 2, Benjamin Cole 2, Neha Malhan 1, Amir H. Ahkami 1, Tanya E. Winkler 1, Joy Hollingsworth 3, Julie Sievert 3, Jeff Dahlberg 3, Robert Hutmacher 4,5, Mary Madera 6, Judith A. Owiti 6, Kim K. Hixson 1, Peggy G. Lemaux 6, Christer Jansson 1, Ljiljana Paša-Tolić 1
1Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute, Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center, University of California Agriculture and Natural Resources, 4West Side Research and Extension Center, University of California, 5Department of Plant Sciences, University of California, Davis, 6Department of Plant and Microbial Biology, University of California, Berkeley
The protocol has been developed to effectively extract intact histones from sorghum leaf materials for profiling of histone post-translational modifications that can serve as potential epigenetic markers to aid engineering drought resistant crops.
