Morgridge Institute for Research, Madison, Wisconsin

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Brian Burkel^1,2, Brett A. Morris¹, Suzanne M. Ponik¹, Kristin M. Riching¹, Kevin W. Eliceiri^2,3,4, Patricia J. Keely^1,2,5

¹Department of Cell and Regenerative Biology, University of Wisconsin-Madison, ²Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, ³Department of Biomedical Engineering, University of Wisconsin-Madison, ⁴Morgridge Institute for Research, University of Wisconsin-Madison, ⁵Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison

Collagen is a core component of the ECM, and provides essential cues for several cellular processes ranging from migration to differentiation and proliferation. Provided here is a protocol for embedding cells within 3D collagen hydrogels, and a more advanced technique for generating randomized or aligned collagen matrices using PDMS microchannels.

Bioengineering

Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae

Kayla Huemer^*1,2, Jayne M. Squirrell^*3, Robert Swader², Kirsten Pelkey², Danny C. LeBert⁴, Anna Huttenlocher^5,6, Kevin W. Eliceiri^1,2,3

¹Department of Biomedical Engineering, University of Wisconsin-Madison, ²Morgridge Institute for Research, University of Wisconsin-Madison, ³Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, ⁴Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison, ⁵Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, ⁶Department of Pediatrics, University of Wisconsin-Madison

This manuscript describes the zWEDGI (zebrafish Wounding and Entrapment Device for Growth and Imaging), which is a compartmentalized device designed to orient and restrain zebrafish larvae. The design permits tail transection and long-term collection of high-resolution fluorescent microscopy images of wound healing and regeneration.

Bioengineering

Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools

Yuming Liu¹, Kevin W. Eliceiri^1,2,3

¹Laboratory for Optical and Computational Instrumentation, Center for Quantitative Cell Imaging, University of Wisconsin-Madison, ²Departments of Medical Physics and Biomedical Engineering, University of Wisconsin-Madison, ³Morgridge Institute for Research, Madison, Wisconsin, ⁴test, test

Here, we present a protocol to use a curvelet transform-based, open-source MATLAB software tool for quantifying fibrillar collagen organization in the extracellular matrix of both normal and diseased tissues. This tool can be applied to images with collagen fibers or other types of line-like structures.