Simons Electron Microscopy Center, New York Structural Biology Center
2 ARTICLES PUBLISHED IN JoVE
Biology
Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton
William C. Budell 1, Luis Allegri 2, Venkat Dandey 1, Clinton S. Potter 1, Bridget Carragher 1
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 2piTree Software
The protocol presented here describes the use of Spotiton, a novel robotic system, to deliver two samples of interest onto a self-wicking, nanowire grid that mix for a minimum of 90 ms prior to vitrification in liquid cryogen.
Biochemistry
Cryo-Electron Microscopy Screening Automation Across Multiple Grids Using Smart Leginon
Anjelique Sawh-Gopal 1,2, Aygul Ishemgulova *1, Eugene Y. D. Chua *1, Mahira F. Aragon *1, Joshua H. Mendez 1, Edward T. Eng 1, Alex J. Noble 1
1Simons Electron Microscopy Center, New York Structural Biology Center, 2Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology
Cryo-electron microscopy (cryoEM) multi-grid screening is often a tedious process that demands hours of attention. This protocol shows how to set up a standard Leginon collection and Smart Leginon Autoscreen to automate this process. This protocol can be applied to the majority of cryoEM holey foil grids.
