Rutgers-New Jersey Medical School
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
Peilin Zheng *1,2, Grant Bertolet *1,2,3, Yuhui Chen 1,2, Shengjian Huang 1,2, Dongfang Liu 1,2,3
1Center for Human Immunobiology, Texas Children's Hospital, 2Department of Pediatrics, Baylor College of Medicine, 3Department of Pathology and Immunology, Baylor College of Medicine
We describe here a combination of the glass-supported lipid bilayer technique of forming immunological synapses with the super-resolution imaging technique of stimulated emission depletion (STED) microscopy. The goal of this protocol is to provide users with the instructions necessary to successfully carry out these two techniques.
Cancer Research
Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line
Minh Ma 1,2, Saiaditya Badeti 1, James K. Kim 1, Dongfang Liu 1,3
1Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey
Here, we present a method to expand peripheral blood natural killer (PBNK), NK cells from liver tissues, and chimeric antigen receptor (CAR)-NK cells derived from peripheral blood mononuclear cells (PBMCs) or cord blood (CB). This protocol demonstrates the expansion of NK and CAR-NK cells using 221-mIL-21 feeder cells in addition to the optimized purity of expanded NK cells.
