Obtainment of Macrophages from Human Monocytes to Assess Leishmania braziliensis Infection Rate and Innate Host Immune ResponseIcaro Bonyek-Silva 1,2, Sara Nunes 1,2, Rana Bastos 1, Reinan Lima 1,2, Leilane Barbosa 1,2, Gabriela Grimaldi 1, Vinicius Rocha 4, Milena B. P. Soares 1,4, Patrícia S T Veras 1,3, Juliana de Menezes 1,2, Cláudia Brodskyn 1,2,3, Natalia Tavares 1,2
1Oswaldo Cruz Foundation, Gonçalo Moniz Institute, 2Federal University of Bahia, 3National Institute of Science and Technology (INCT), Research Institute in Immunology, 4Senai Institute for Innovation in Advanced Health Systems, National Service for Industrial Learning, Integrated Manufacturing and Technology Campus, SENAI - CIMATEC Salvador
This protocol describes the process for obtaining human macrophages from monocytes for infection with Leishmania braziliensis. It also allows researchers to evaluate infection rate and parasite viability, ROS production by fluorescence microscopy, and the production of inflammatory mediators in culture supernatants to investigate macrophage response to infection.
