Optical Tweezers to Study RNA-Protein Interactions in Translation RegulationLukas Pekarek 1, Stefan Buck 1, Neva Caliskan 1,2
1Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research), 2Medical Faculty, Julius-Maximilians University Würzburg
This protocol presents a complete experimental workflow for studying RNA-protein interactions using optical tweezers. Several possible experimental setups are outlined including the combination of optical tweezers with confocal microscopy.
