International Max-Planck Research School for Biology and Computation

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

Paul-Lennard Mendez^1,2, Leon Obendorf¹, Petra Knaus¹

¹Institute for Chemistry and Biochemistry, Freie Universität Berlin, ²International Max-Planck Research School for Biology and Computation

Here, we establish a protocol to simultaneously visualize and analyze multiple SMAD complexes using proximity ligation assay (PLA) in endothelial cells exposed to pathological and physiological fluid shear stress conditions.