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Nanjing Advanced Academy of Life and Health

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Xiaoyan Chang^*1, Keru Zhou^*1, Yanguang Liu^1,2, Lian Duan³, Lichun Zhang², Guoliang Zhang³, Haikun Wang⁴, Guiqin Wang¹

¹Nanjing Advanced Academy of Life and Health, ²Institute of Animal Bio-technology, Jilin Academy of Agricultural Sciences, ³National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital, Southern University of Science and Technology, ⁴CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences

Here we describe a protocol for pseudovirus packaging and the measurement of antibody neutralizing activity.

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