Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory
3 ARTICLES PUBLISHED IN JoVE
Neuroscience
An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
Nastasia K-H Lim 1,2, Visse Moestrup 3,4, Xiao Zhang 1, Wen-An Wang 5, Arne Møller 3,4,6, Fu-De Huang 1,4
1Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Science, 2Shanghai Institute of Materia Medica, University of Chinese Academy of Sciences, Chinese Academy of Science, 3Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 4Sino-Danish Center for Education and Research (SDC), 5Department of Neurology, Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiao Tong University School of Medicine, 6Department of Nuclear Medicine and PET-centre, Aarhus University Hospital
This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal cord.
Medicine
Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells
Xiao Zhang 1, Zi-Bing Jin 1
1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory
Using a self-organizing method, we develop a protocol with the addition of COCO that could significantly increase the generation of photoreceptors.
Cancer Research
Reconstruct Human Retinoblastoma In Vitro
Xiao Zhang 1, Zi-Bing Jin 1
1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory
We describe a method for generating human retinoblastoma (RB) by introducing biallelic RB1 mutations in human embryonic stem cells (hESC). RB cell lines could also be successfully cultured using the isolated RB in a dish.
