Morgridge Institute for Research, Madison, WI
2 ARTICLES PUBLISHED IN JoVE
Biology
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
Michael Weber 1, Michaela Mickoleit 1, Jan Huisken 1
1Huisken Lab, Max Planck Institute of Molecular Cell Biology and Genetics
The development of zebrafish can be followed over days with light sheet microscopy when embryos are embedded in optically clear polymer tubes with low-concentration agarose.
Biology
Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos
Anjalie Schlaeppi 1, Alyssa Graves 1, Michael Weber 1, Jan Huisken 1
1Morgridge Institute for Research, Madison, WI
We describe optimized tools to study the zebrafish heart in vivo with light sheet fluorescence microscopy. Specifically, we suggest bright cardiac transgenic lines and present new gentle embedding and immobilization techniques that avoid developmental and heart defects. A possible data acquisition and analysis pipeline adapted to cardiac imaging is also provided.
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