Luoyang TMAXTREE Biotechnology Co., Ltd.

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)

Xingjin Jian^*1,2, Xiaojie Guo^*1,2,3, Jia Wang⁴, Zheng Lin Tan^1,2,6, Xin-hui Xing^1,2,5, Liyan Wang³, Chong Zhang^1,2,5

¹Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, ²Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, ³Luoyang TMAXTREE Biotechnology Co., Ltd., ⁴Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, ⁵Center for Synthetic & Systems Biology, Tsinghua University, ⁶School of Life Science and Technology, Tokyo Institute of Technology

This protocol describes how to use the Microbial Microdroplet Culture system (MMC) to conduct automated microbial cultivation and adaptive evolution. MMC can cultivate and sub-cultivate microorganisms automatically and continuously and monitor online their growth with relatively high throughput and good parallelization, reducing labor and reagent consumption.

Bioengineering

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using The Single-cell Microliter-droplet Culture Omics System

Wenhsuan Chou^1,2, Xiaojie Guo³, Liyan Wang^3,4, Xin-Hui Xing^1,5,6,7, Yi Wang^1,2, Chong Zhang^1,2,5

¹Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, ²Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, ³Luoyang TMAXTREE Biotechnology Co., Ltd., ⁴Biobreeding Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, ⁵Center for Synthetic and Systems Biology, Tsinghua University, ⁶Institute of Biomedical Health Technology and Engineering, Shenzhen Bay laboratory, ⁷Institute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University

This protocol describes how to use the Single-cell Microliter-droplet Culture Omics System (MISS cell) to perform microbial monoclonal isolation, cultivation, and picking. The MISS cell achieves an integrated workflow based on droplet microfluidic technology, which offers excellent droplet monodispersity, high parallel cultivation, and high-throughput biomass detection.