The Second Hospital of Tianjin Medical University
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
Huiqin Tang *1, Hui Xie *1, Zhun Wang 1, Shuanghe Peng 2, Wenli Ni 1, Linpei Guo 3
1Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, 2Department of Pathology, The Second Hospital of Tianjin Medical University, 3Department of Urology, The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University
Here, we present an economical and efficient method to isolate and generate high-purity bone marrow-derived dendritic cells from mice after 7 days of culture with 10 ng/mL GM-CSF/IL-4.
Cancer Research
Establishing a Murine Superficial Bladder Cancer Model via an Intravesical Cell Administration Technique
Zhe Zhang 1,2, Zhouliang Wu 1,2, Chong Shen 1,2, Zhiheng Liu 2, Hailong Hu 1,2
1Department of Urology, The Second Hospital of Tianjin Medical University, 2Tianjin Key Laboratory of Urology, Tianjin Institute of Urology
This protocol describes a unique method for constructing an orthotopic model of superficial bladder cancer.
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