Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

Victoria Lucia Alonso^1,2, Romina Manarin¹, Virginia Perdomo¹, Ernesto Gulin³, Esteban Serra^1,2, Pamela Cribb^1,2

¹Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), ²Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, ³Instituto de Investigaciones Biomédicas (INBIOMED), Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

We describe a high-throughput colorimetric assay measuring β-galactosidase activity in three life cycle stages of Trypanosoma cruzi, the causative agent of Chagas disease. This assay can be used to identify trypanocidal compounds in an easy, fast, and reproducible manner.