University of Wrocław
2 ARTICLES PUBLISHED IN JoVE
Cancer Research
Gelatin Zymography to Detect Gelatinase Activity in Melanoma Cells
Ewa Mrówczyńska 1, Ewa Mazurkiewicz 1, Antonina Joanna Mazur 1
1Department of Cell Pathology, Faculty of Biotechnology, University of Wrocław
Metalloproteases (MMPs) are secreted by many cells, including malignant melanoma. MMP-mediated cleavage of extracellular matrix components leads to the increased invasive potential of these cells. Gelatin zymography, presented here, is a quantifying method for studying gelatinase activity manifested as a digested gelatin area on a polyacrylamide gel.
Cancer Research
Isolation of Stably Transfected Melanoma Cell Clones
Ewa Mazurkiewicz 1, Ewa Mrówczyńska 1, Antonina Joanna Mazur 1
1Department of Cell Pathology, Faculty of Biotechnology, University of Wrocław
A protocol is described for isolating stably transfected melanoma cell clones using glass cylinders. We focus on practical advice that may be decisive for the success of the entire procedure.
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