Norris Cotton Cancer Center

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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Kali A. Smolen¹, Arminja N. Kettenbach^1,2

¹Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, ²Norris Cotton Cancer Center

Here, we present a protocol for the enrichment of endogenous phosphoprotein phosphatases and their interacting proteins from cells and tissues and their identification and quantification by mass spectrometry-based proteomics.