IEO, European Institute of Oncology IRCCS

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Giulia Ercoli¹, Gianluca Lopez^1,2, Camilla Ciapponi^1,3, Chiara Corti^1,2, Luca Despini⁴, Donatella Gambini⁵, Letterio Runza¹, Concetta Blundo⁴, Amedeo Sciarra¹, Nicola Fusco^1,6

¹Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ²School of Medicine, University of Milan, ³School of Biology, University of Pavia, ⁴Division of Breast Surgery, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, ⁵Division of Medical Oncology, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, ⁶Department of Biomedical, Surgical, and Dental Sciences, University of Milan

Heterogeneous distribution of HER2-positive cells can be observed in a subset of breast cancers and generates clinical dilemmas. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare HER2 intra-tumor genetic heterogeneity in a large series of heterogeneously processed breast cancers.

Medicine

Integrating Augmented Reality Tools in Breast Cancer Related Lymphedema Prognostication and Diagnosis

Marco Invernizzi¹, Letterio Runza², Alessandro De Sire¹, Lorenzo Lippi¹, Concetta Blundo³, Donatella Gambini⁴, Renzo Boldorini^5,6, Stefano Ferrero^2,7, Nicola Fusco^2,7

¹Physical and Rehabilitative Medicine, Department of Health Sciences, University of Eastern Piedmont "A. Avogadro", ²Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ³Division of Breast Surgery, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ⁴Division of Medical Oncology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ⁵Division of Pathology, Maggiore della Carità Hospital, ⁶Department of Health Sciences, University of Eastern Piedmont "A. Avogadro", ⁷Department of Biomedical, Surgical, and Dental Sciences, University of Milan

Breast cancer related lymphedema is frequent in breast cancer survivors, but there are not widely employed guidelines for its diagnosis and quantification. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare upper limb volume in breast cancer patients.

Cancer Research

Biobank for Translational Medicine: Standard Operating Procedures for Optimal Sample Management

Giuseppina Bonizzi^*1, Maria Capra^*1, Cristina Cassi¹, Giulio Taliento¹, Oriana Pala², Elham Sajjadi^2,3, Konstantinos Venetis^2,3, Mariia Ivanova², Massimo Monturano⁴, Giuseppe Renne², Lorenzo Zattoni^1,3, Elena Guerini-Rocco^2,3, Giuseppe Viale^2,3, Roberto Orecchia⁵, Nicola Fusco^1,2,3

¹Biobank for Translational and Digital Medicine Unit, IEO, European Institute of Oncology IRCCS, ²Division of Pathology, IEO, European Institute of Oncology IRCCS, ³Department of Oncology and Hemato-Oncology, University of Milan, ⁴Patient Safety & Risk Management Service, IEO, European Institute of Oncology IRCCS, ⁵Scientific Directorate, IEO, European Institute of Oncology IRCCS

Biobanks are crucial resources for biomedical research and the Biobank for Translational and Digital Medicine Unit at the European Institute of Oncology is a model in this field. Here, we provide a detailed description of biobanks' standard operating procedures for the management of different types of human biological samples.