Shandong Provincial Hospital Affiliated to Shandong First Medical University

5 ARTICLES PUBLISHED IN JoVE

Biology

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

Hui Zhang^1,2, Niko G. Gubernator^3,4, Minerva Yue¹, Roland G. W. Staal¹, Eugene V. Mosharov¹, Daniela Pereira¹, Vojtech Balsanek³, Paul A. Vadola³, Bipasha Mukherjee⁵, Robert H. Edwards⁵, David Sulzer^1,2,6, Dalibor Sames³

¹Departments of Neurology, Columbia University, ²Departments of Psychiatry and Pharmacology, Columbia University, ³Department of Chemistry, Columbia University, ⁴eMolecules, Inc., ⁵Departments of Neurology and Physiology, University of California School of Medicine, San Francisco, ⁶Division of Molecular Therapeutics, New York Psychiatric Institute

A new means to measure neurotransmission optically using fluorescent dopamine analogs.

Cancer Research

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

Xiaoyu An^*1,2, Xuesong Ouyang^*1, Hui Zhang¹, Tingting Li¹, Yu-yang Huang¹, Zhiyuan Li¹, Demin Zhou², Qi-Xang Li^1,2

¹Crown Bioscience Inc., ²State Key Laboratory of Natural and Biomimetic Drugs, Peking University

The experimental procedure on the immunophenotyping of murine orthotopic PDAC homografts aims at profiling the tumor immuno-microenvironment. Tumors are orthotopically implanted via surgery. Tumors of 200–600 mm³ in size were harvested and dissociated to prepare single-cell suspensions, followed by multi-immune marker FACS analysis using different fluorescently-labeled antibodies.

Neuroscience

Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice

Lianteng Zhi¹, Jingyu Zhao¹, David Jaffe¹, Yuanxin Chen¹, Ninghan Wang^1,2, Qi Qin¹, Erin L. Seifert³, Chenjian Li⁴, Hui Zhang¹

¹Department of Neuroscience, Thomas Jefferson University, ²School of Biomedical Engineering, Drexel University, ³Department of Pathology, MitoCare Center, Thomas Jefferson University, ⁴School of Life Sciences, Peking University

Oxygen consumption rate (OCR) is a common proxy for mitochondrial function and can be used to study different disease models. We developed a new method using a Seahorse XF analyzer to directly measure the OCR in acute striatal slices from adult mice that is more physiologically relevant than other methods.

Medicine

Extraperitoneal Laparoscopic Modified Y-V Plasty for the Treatment of Refractory Bladder Neck Contracture

Zhenjie Zang¹, Dingchang Shao¹, Hui Zhang², Dingqi Sun², Tongxiang Diao², Keqin Zhang², Qiang Fu^1,2

¹Department of Urology, Shandong Provincial Hospital, Shandong University, ²Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University

Here, we describe a modified surgical procedure for refractory bladder neck contracture that is associated with easy manipulation, optimum intraoperative exposure, and minimal invasiveness.

Biology

Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model

Yanjing Guo^1,2,3, Wei Li^2,3, Yinghan Wang²

¹The Affiliated Hospital of Qingdao University, ²Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, ³Department of Anesthesiology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University

Follicle-stimulating hormone (FSH) in various extragonadal tissues and organs is associated with the pathogenesis of multiple diseases. The ovariectomized and FSH-treated mouse model (OVF) can be used to explore the extragonadal actions of FSH.