University of Maryland Institute for Bioscience and Biotechnology Research
2 ARTICLES PUBLISHED IN JoVE
Genetics
Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis
Isabelle Louradour 1, Kashinath Ghosh 1, Ehud Inbar 1, David L. Sacks 1, Channa Aluvihare 2, Robert A. Harrell II 2
1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2University of Maryland Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research
This protocol details the steps of CRISPR/Cas9 targeted mutagenesis in sand flies: embryo collection, injection, insect rearing, and identification as well as selection of mutations of interest.
Biology
Embryo Injection Technique for Gene Editing in the Black-Legged Tick, Ixodes scapularis
Arvind Sharma 1, Michael Pham 1, Robert A. Harrell II 2, Andrew B. Nuss 3, Monika Gulia-Nuss 1
1Department of Biochemistry and Molecular Biology, University of Nevada, Reno, 2Insect Transformation Facility, University of Maryland Institute for Bioscience and Biotechnology Research, 3Department of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno
The present protocol describes a method for injecting tick embryos. Embryo injection is the preferred technique for genetic manipulation to generate transgenic lines.
