The University of Arizona, Tucson

2 ARTICLES PUBLISHED IN JoVE

Medicine

Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies

Hannah E. Korah¹, Kevin Cheng¹, Stephanie M. Washington², Matthew E. Flowers¹, Harrison J. Stratton¹, Amol Patwardhan⁵, Mohab M. Ibrahim^*1,2,3,4, Laurent F. Martin^*1,2

¹Department of Pharmacology, College of Medicine, The University of Arizona, Tucson, ²Department of Anesthesiology, College of Medicine, The University of Arizona, Tucson, ³Neuroscience Graduate Interdisciplinary Program, College of Medicine, The University of Arizona, Tucson, ⁴Comprehensive Pain and Addiction Center, The University of Arizona, Tucson, ⁵Department of Anesthesiology and Pain Management, University of Texas Southwestern Medical Center

Partial sciatic nerve ligation induces long-lasting chronic neuropathic pain, characterized by exaggerated responses to thermal and mechanical stimuli. This mouse model of neuropathic pain is commonly used to study innovative therapies for pain management. This article describes in detail the surgical procedure to improve standardization and reproducibility.

Medicine

Optimizing Photoneuromodulation Techniques to Evaluate the Role of Green Light-Emitting Diodes in Pain Management

Khaled A. Ismail¹, Victoria Curfman¹, Joon Park¹, Emma Marie Louis^1,2, Hannah Korah², Stephanie Marie Washington¹, Mohab M. Ibrahim^*1,2,3, Laurent F. Martin^*1,2,3

¹Department of Anesthesiology, College of Medicine, The University of Arizona, Tucson, ²Department of Pharmacology, College of Medicine, The University of Arizona, Tucson, ³Comprehensive Center for Pain and Addiction, College of Medicine, The University of Arizona, Tucson

Recent developments in pain research highlight the potential of photoneuromodulation using green light-emitting diodes (GLED) as a non-pharmacological treatment. GLED modulates pain pathways, offering effective pain relief. This article aims to standardize and refine GLED exposure protocols, improving consistency across studies and advancing the clinical application of this therapy.