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The Institute of Cancer Research

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Three-Dimensional (3D) Tumor Spheroid Invasion Assay
Maria Vinci 1,2, Carol Box 2, Suzanne A. Eccles 2
1Division of Molecular Pathology, The Institute of Cancer Research, 2Division of Cancer Therapeutics, The Institute of Cancer Research

Invasion of surrounding normal tissues is a defining characteristic of malignant tumors. We provide here a simple, semi-automated micro-plate assay of invasion into a natural 3D biomatrix that has been exemplified with a number of models of advanced human cancers.

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Cancer Research

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
Eamonn Morrison 1,2, Patty Wai 1,2, Andri Leonidou 1,2, Philip Bland 1,2, Saira Khalique 1,2, Gillian Farnie 3, Frances Daley 1, Barrie Peck 1,2, Rachael Natrajan 1,2
1The Breast Cancer Now Toby Robins Research Centre, Division of Breast Cancer, The Institute of Cancer Research, 2Division of Molecular Pathology, The Institute of Cancer Research, 3Institute of Cancer Sciences, University of Manchester

Identifying novel drug targets that transition from pre-clinical testing to human trials is a scientific priority. To that end, here we describe a functional genomics approach for examining the impact of gene depletion on cancer cell line spheroids, which more appropriately model human cancers in vivo.

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Biology

Measuring the Confluence of iPSCs Using an Automated Imaging System
Valentina Magliocca 1,2, Maria Vinci 3, Tiziana Persichini 2, Franco Locatelli 3, Marco Tartaglia 1, Claudia Compagnucci 1
1Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS, 2Department of Science, University Roma Tre, 3Department of Onco-hematology, Gene and Cell Therapy, Ospedale Pediatrico Bambino Gesù, IRCCS

The goal of the protocol is to compare different extracellular matrix (ECM) coating conditions to assess how differential coating affects the growth rate of induced pluripotent stem cells (iPSCs). In particular, we aim to set up conditions to obtain optimal growth of iPSC cultures.

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