Slovak Academy of Sciences

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

Takahiro Tadokoro¹, Atsushi Miyanohara¹, Michael Navarro¹, Kota Kamizato¹, Stefan Juhas², Jana Juhasova², Silvia Marsala¹, Oleksandr Platoshyn¹, Erik Curtis³, Brandon Gabel³, Joseph Ciacci³, Nada Lukacova⁴, Katarina Bimbova⁴, Martin Marsala^1,4

¹Neuroregeneration Laboratory, Department of Anesthesiology, University of California, San Diego, ²Institute of Animal Physiology and Genetics, Czech Academy of Sciences, ³Department of Neurosurgery, University of California, San Diego, ⁴Institute of Neurobiology, Slovak Academy of Sciences

The goal of the present study was to develop and validate the potency and safety of spinal adeno-associated virus 9 (AAV9)-mediated gene delivery by using a novel subpial gene delivery technique in adult mice.

Biology

A Versatile Model of Hard Tick Infestation on Laboratory Rabbits

Consuelo Almazán¹, Sarah Bonnet¹, Martine Cote¹, Mirko Slovák², Yoonseong Park³, Ladislav Šimo¹

¹Unité Mixte de Recherche (UMR) Biologie moléculaire et immunologie parasitaires (BIPAR), Institut national de la recherche agronomique (INRA), Ecole Nationale Vétérinaire d'Alfort, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Paris-Est, ²Institute of Zoology, Slovak Academy of Sciences, ³Department of Entomology, Kansas State University

We have developed a simple and versatile system to feed hard ticks on laboratory rabbits. Our non-laborious protocol uses easily accessible materials and can be adjusted depending on the requirements of the various experimental settings. The method allows comfortable monitoring and/or sampling of ticks during the entire feeding period.

Chemistry

Demonstrating the Simplicity and In Situ Temperature Monitoring of the Mechanochemical Synthesis of Metal Chalcogenides Suitable for Thermoelectrics

Imelda Octa Tampubolon^1,2, Patrik Jacko³, Matej Bereš³, Peter Baláž¹, Matej Baláž¹

¹Institute of Geotechnics, Slovak Academy of Sciences, ²Faculty of Materials, Metallurgy and Recycling, Technical University of Košice, ³Faculty of Electrical Engineering and Informatics, Technical University of Košice

Here, we present a protocol to synthesize two metal chalcogenides (Cu_1.8S and SnSe) suitable for thermoelectrics via an ultrafast (second-range), solvent-free, and one-step mechanochemical synthesis using elemental precursors. Simultaneously, we demonstrate the monitoring of the temperature in the jar during planetary ball milling in situ by the newly developed device.