University of Toledo

4 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Marcus L. Basiri¹, Stephanie Blachon¹, Yiu-Cheung Frederick Chim¹, Tomer Avidor-Reiss¹

¹Department of Biological Sciences, University of Toledo

Imaging of centrosomal proteins during Drosophila spermatogenesis is a powerful method to identify new proteins critical for centrosome biology as well as to elucidate the particular function of known players in this process.

Chemistry

Achieving Moderate Pressures in Sealed Vessels Using Dry Ice As a Solid CO₂ Source

Mohit Kapoor^1,2, Pratibha Chand-Thakuri^*1,2, Justin M. Maxwell^*1,2, Michael C. Young^1,2

¹Department of Chemistry and Biochemistry, University of Toledo, ²School of Green Chemistry and Engineering, University of Toledo

Here we present a protocol for performing reactions in simple reaction vessels under low-to-moderate pressures of CO₂. The reactions can be performed in a variety of vessels simply by administering the carbon dioxide in the form of dry ice, without the need for costly or elaborate equipment or set-ups.

Biochemistry

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Heidi L. Fresenius¹, Matthew L. Wohlever¹

¹Department of Chemistry & Biochemistry, University of Toledo

Here, we present a detailed protocol for reconstitution of Msp1 extraction activity with fully purified components in defined proteoliposomes.

Bioengineering

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Qingliang Yang¹, Jordan C. Langston², Yuan Tang³, Balabhaskar Prabhakarpandian⁴, Laurie E. Kilpatrick⁵, Mohammad F. Kiani^1,2,6

¹Department of Mechanical Engineering, Temple University, ²Department of Bioengineering, Temple University, ³Department of Bioengineering, University of Toledo, ⁴Biomedical Technology, CFD Research Corporation, ⁵Center for Inflammation and Lung Research, Department of Microbiology, Immunology and Inflammation, Lewis Katz School of Medicine, Temple University, ⁶Department of Radiation Oncology, Lewis Katz School of Medicine, Temple University

In this protocol, a biomimetic microfluid assay, which can reproduce a physiologically relevant microvascular environment and reproduce the entire leukocyte adhesion/migration cascade, is employed to study leukocyte-endothelial cell interactions in inflammatory disease.