Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick GutToru Kariu 1, Adam S. Coleman 1, John F. Anderson 2, Utpal Pal 1
1Department of Veterinary Medicine, University of Maryland, 2Department of Entomology, Connecticut Agricultural Experiment Station
Lyme disease research studies often require generation of ticks infected with the pathogen Borrelia burgdorferi, a process that typically takes several weeks. Here we demonstrate a microinjection-based tick infection procedure that can be accomplished within hours. We also demonstrate an immunofluorescence method for in situ localization of B. burgdorferi within ticks.
