February 14th, 2011
•Lyme disease research studies often require generation of ticks infected with the pathogen Borrelia burgdorferi, a process that typically takes several weeks. Here we demonstrate a microinjection-based tick infection procedure that can be accomplished within hours. We also demonstrate an immunofluorescence method for in situ localization of B. burgdorferi within ticks.
Related Videos
'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research
Rescue of Recombinant Newcastle Disease Virus from cDNA
Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples
Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model
Immunohistochemistry Test for the Lyssavirus Antigen Detection from Formalin-Fixed Tissues
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved