Healthcare Research Institute Princesa Hospital (IIS-Princesa)

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

T Cells Capture Bacteria by Transinfection from Dendritic Cells

Aranzazu Cruz-Adalia¹, Guillermo Ramírez-Santiago^1,2, Mónica Torres-Torresano^1,2, Raquel Garcia-Ferreras^1,2, Esteban Veiga Chacón¹

¹Cellular and Molecular Biology, Spanish National Research Council (CSIC), Spanish National Biotechnology Centre (CNB-CSIC), ²Hospital de Santa Cristina, Healthcare Research Institute Princesa Hospital (IIS-Princesa)

Here a protocol is presented to measure bacterial capture by CD4⁺ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.