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T Cells Capture Bacteria by Transinfection from Dendritic Cells

DOI :

10.3791/52976-v

January 13th, 2016

January 13th, 2016

11,483 Views

1Cellular and Molecular Biology, Spanish National Research Council (CSIC), Spanish National Biotechnology Centre (CNB-CSIC), 2Hospital de Santa Cristina, Healthcare Research Institute Princesa Hospital (IIS-Princesa)

Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.

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