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Indiana University Melvin and Bren Simon Cancer Center

4 ARTICLES PUBLISHED IN JoVE

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Medicine

Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of β-aminopropionitrile-induced Aortic Aneurysm and Dissection
Brittany O. Aicher 1, Subhradip Mukhopadhyay 1, Xin Lu 2, Selen C. Muratoglu 1, Dudley K. Strickland 1, Areck A. Ucuzian 1,3
1Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, 2Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, 3Division of Vascular Surgery, University of Maryland School of Medicine

This article describes a detailed methodology of using a radiopaque lead-based silicone rubber to perfuse the murine vasculature for aortic diameter quantification in a mouse model of aortic aneurysm and dissection.

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Biology

A Proximal Culture Method to Study Paracrine Signaling Between Cells
Subramanyam Dasari 1, Taruni Pandhiri 1, James Haley 1, Dean Lenz 2, Anirban K. Mitra 1,3,4
1Indiana University School of Medicine, 2Urology, Indiana University Health Southern Indiana Physicians, 3Indiana University Melvin and Bren Simon Cancer Center, 4Department of Medical and Molecular Genetics, Indiana University School of Medicine

Paracrine and juxtacrine cellular interactions play an important role in many biological processes, including tumor progression, immune responses, angiogenesis, and development. Here, a proximal culture method is used to study paracrine signaling where the localized concentrations of the secreted factors are maintained while preventing direct cellular contact.

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Cancer Research

An Ivor Lewis Esophagectomy Designed to Minimize Anastomotic Complications and Optimize Conduit Function
Neal K. Ramchandani 1, Kenneth A. Kesler 1, Jonathon D. Rogers 1, Nakul Valsangkar 1, Samatha M. Stokes 1, Shadia I. Jalal 2
1Department of Surgery, Division of Cardiothoracic, Indiana University Melvin and Bren Simon Cancer Center, 2Department of Medicine, Medical Oncology Division, Indiana University Melvin and Bren Simon Cancer Center

We describe a novel intrathoracic esophagogastric anastomotic technique designed to create a large diameter anastomosis while simultaneously maintaining conduit blood supply to minimize the incidence of anastomotic leaks and strictures. Construction and orientation of the stomach conduit designed to optimize upper gastrointestinal tract function is also described.

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Biochemistry

Nitropeptide Profiling and Identification Illustrated by Angiotensin II
Shan Feng 1,2, Xiaofei Wen 3, Xin Lu 2,4,5,6
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

Proteomic profiling of tyrosine-nitrated proteins has been a challenging technique due to the low abundance of the 3-nitrotyrosine modification. Here we describe a novel approach for nitropeptide enrichment and profiling by using Angiotensin II as the model. This method can be extended for other in vitro or in vivo systems.

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