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A Proximal Culture Method to Study Paracrine Signaling Between Cells

DOI :

10.3791/58144-v

August 28th, 2018

August 28th, 2018

9,226 Views

1Indiana University School of Medicine, 2Urology, Indiana University Health Southern Indiana Physicians, 3Indiana University Melvin and Bren Simon Cancer Center, 4Department of Medical and Molecular Genetics, Indiana University School of Medicine

Paracrine and juxtacrine cellular interactions play an important role in many biological processes, including tumor progression, immune responses, angiogenesis, and development. Here, a proximal culture method is used to study paracrine signaling where the localized concentrations of the secreted factors are maintained while preventing direct cellular contact.

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