Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living CellsHonggun Lee 1, Constantin Radu 1, Jeung Whan Han 2, Regis Grailhe 3
1Automation & Logistics Management, Screening Sciences & Novel Assay Technologies, Institut Pasteur Korea, 2School of Pharmacy, Sungkyunkwan University, 3Technology Development Platform, Institut Pasteur Korea
We describe a method to quantify the aggregation of misfolded proteins. Our protocol details lentiviral induced stable cell line generation, automated confocal imaging, and image analysis of protein aggregates. As an illustrative application, we studied the effect of small molecules in promoting SOD1 aggregation in a time- and dose-dependent manner.
