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Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

DOI :

10.3791/56425-v

October 4th, 2017

October 4th, 2017

7,028 Views

1Automation & Logistics Management, Screening Sciences & Novel Assay Technologies, Institut Pasteur Korea, 2School of Pharmacy, Sungkyunkwan University, 3Technology Development Platform, Institut Pasteur Korea

We describe a method to quantify the aggregation of misfolded proteins. Our protocol details lentiviral induced stable cell line generation, automated confocal imaging, and image analysis of protein aggregates. As an illustrative application, we studied the effect of small molecules in promoting SOD1 aggregation in a time- and dose-dependent manner.

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Assay Development

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