National Jewish Health and University of Colorado School of Medicine

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Lesly De Arras¹, Brandon S. Guthrie¹, Scott Alper¹

¹Integrated Department of Immunology and Integrated Center for Genes, Environment, and Health, National Jewish Health and University of Colorado School of Medicine

In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine production.