November 3rd, 2014
•In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine production.
Related Videos
RNA Interference in Ticks
An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
Quantitative Measurement of the Immune Response and Sleep in Drosophila
Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish
Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection
Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved