Investigating Teliospore Germination Using Microrespiration Analysis and MicrodissectionLauren A. Ostrowski *1, Amanda M. Seto *1, Barry Saville 1,2
1Environmental and Life Sciences Graduate Department, Trent University, 2Forensic Science Department, Trent University
Smut fungi cause many devastating agricultural diseases. They are dispersed as dormant teliospores that germinate in response to environmental cues. We outline two methods to investigate molecular changes during germination: measuring respiration increase to detect metabolic activation and assessing changing molecular events by isolating teliospores at distinct morphological stages.
