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This video describes in vivo electroporation, which is a technique for DNA delivery into ependymoglial cells in adult zebrafish.
1. Electroporation
Figure 1: Schematic representation of coronal section of the everted zebrafish telencephalon. (A) Scheme of a coronal section of zebrafish telencephalon, highlighting the position of ependymoglial cells, which are lining ventricular surface and building the ventral ventricular wall. Dorsal ependymal layer is bridging the two hemispheres and covering the ventricle (V), located in between two cell...
Name | Company | Catalog Number | Comments |
Ultrasound gel | SignaGel, Parker laboratories INC | 15-60 | Electrode Gel |
BTX Tweezertrodes Electrodes | Platinum Tweezertrode, BTX Harvard Apparatus | 45-0486 | 1mm diameter |
Electroporation device | BTX ECM830 Square Wave Electroporation System, BTX Harvard Apparatus | 45-0662 |
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Source: Durovic, T., et al. Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon. J. Vis. Exp. (2019).
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