Erratum: Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

摘要

A correction was made to Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research. In Protocol section 1.1, the order of the listed chemicals MeOH:H₂O₂:DMSO has accidentally been switched and should instead be MeOH:DMSO:H₂O₂.

摘要

A correction was made to Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research. In Protocol section 1.1, the order of the listed chemicals MeOH:H₂O₂:DMSO has accidentally been switched and should instead be MeOH:DMSO:H₂O₂.

Protocol section 1.1 was changed from:

Incubate the tissue in freshly prepared MeOH:H₂O₂:DMSO bleaching buffer in a 2:1:3 ratio at RT for 24 hr to quench endogenous tissue fluorescence. For larger samples, exchange to new bleaching buffer and incubate for another 24 hr.

to:

Incubate the tissue in freshly prepared MeOH:DMSO:H₂O₂ bleaching buffer in a 2:1:3 ratio at RT for 24 hr to quench endogenous tissue fluorescence. For larger samples, exchange to new bleaching buffer and incubate for another 24 hr.

研究方案

A correction was made to Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research. In Protocol section 1.1, the order of the listed chemicals MeOH:H₂O₂:DMSO has accidentally been switched and should instead be MeOH:DMSO:H₂O₂.

Protocol section 1.1 was changed from:

Incubate the tissue in freshly prepared MeOH:H₂O₂:DMSO bleaching buffer in a 2:1:3 ratio at RT for 24 hr to quench endogenous tissue fluorescence. For larger samples, exchange to new bleaching buffer and incubate for another 24 hr.

to:

Incubate the tissue in freshly prepared MeOH:DMSO:H₂O₂ bleaching buffer in a 2:1:3 ratio at RT for 24 hr to quench endogenous tissue fluorescence. For larger samples, exchange to new bleaching buffer and incubate for another 24 hr.