October 26th, 2009
•We present protocols for the 3-dimensional (3D) encapsulation of cells within synthetic hydrogels. The encapsulation procedure is outlined for two commonly used methods of crosslinking (michael-type addition and light-initiated free radical mechanisms), as well as a number of techniques for assessing encapsulated cell behavior.
Related Videos
Experimental Approaches to Tissue Engineering
Title Cell Encapsulation by Droplets
Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
PuraMatrix Encapsulation of Cancer Cells
Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels
A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid
Rat Mesentery Exteriorization: A Model for Investigating the Cellular Dynamics Involved in Angiogenesis
Mechanical Stimulation of Chondrocyte-agarose Hydrogels