microfluidic shear apparatus setup for single-cell fluid shear assay

Enhancing Targeted Cancer Diagnosis by Quantifying Single-Cell Mechanics

Studying the biophysical differences between cancerous and non-cancerous cells allows for novel diagnostic and therapeutic opportunities1. Understanding how differences in biomechanics/mechanobiology contribute to tumor progression and treatment resistance will reveal new avenues for targeted therapy and early diagnosis2.
While it is known that cancer cell mechanical properties differ from normal cells (e.g., viscoelasticity of the plasma membrane and nuclear envelope)3,4,5, robust and reproducible methods for measuring these properties in live cells are lacking6. The shear assay method is used to quantify the mechanical properties of cells by subjecting single cells to fluid shear stress and analyzing their individual responses and resistance to the applied stress3,4,5,7,8,9. Although several methods and techniques have been used to characterize the mechanical properties of single cells, these tend to affect cell material properties by i) perforating/damaging the cell membrane due to the indentation depth, complex tip geometries, or substrate stiffening associated with atomic force microscopy (AFM)10,11, ii) inducing cellular photodamage during optical trapping12,13, or iii) inducing complex stress states associated with micropipette aspiration14,15. These external effects are associated with significant uncertainties in the accuracy of cell viscoelasticity measurements6,16,17.
To address these limitations, the shear assay method described here provides a highly controllable and simple approach to simulate physiological flow in the body without affecting cellular material properties in the process. Fluid shear stresses in this assay represent mechanical stresses experienced by cells in the body either by fluids within the tumor interstitium or in the blood during circulation18,19,20. Further, these fluid stresses promote various malignant behaviors in cancer cells, including progression, migration, metastasis, and cell death19,21,22,23&#160;which vary between tumorigenic and non-tumorigenic cells. Moreover, the altered mechanical features of cancer cells (i.e., they are often &#34;softer&#34; than normal cells found within the same organ) allow them to persist in hostile tumor microenvironments, invade surrounding normal tissues, and metastasize to distant sites24,25,26. By creating a pseudo-biological environment where cells experience physiological levels of fluid shear stress, a process that is physiologically relevant and not destructive to the cell is achieved. The cellular responses to these applied fluid shear stresses allow us to characterize cell mechanical properties.
This paper provides a shear assay protocol for the extensive study of the mechanical properties and behavior of cancerous and non-cancerous cells under applied shear stress. Cells respond to external forces in an elastic and viscous manner and can therefore be idealized as a viscoelastic material3. This technique is categorized into: (i) cell culture of dispersed single cells, (ii) controlled application of fluid shear stress, (iii) in situ imaging and observation of cellular behavior (including resistance to stress and deformation), (iv) strain analysis of cells to determine the extent of deformation, and (v) characterization of the viscoelastic properties of single cells. By interrogating these mechanical properties and behaviors, complex cellular mechanobiology can be distilled to quantifiable data. A protocol outlining this method allows for the cataloging of and comparison between various malignant and non-malignant cell types. Quantifying these differences has the potential to establish diagnostic and therapeutic biomarkers.

Author Spotlight: Shear Assay Protocol for the Determination of Single-Cell Material Properties  

Shear Assay Protocol for the Determination of Single-Cell Material Properties

The physical hallmarks of cancer are relatively new and exciting topics in cancer research. Our research seeks to specifically characterize the mechanical properties of both cancers and non-cancer cells to better understand the physical mechanisms by which cancer cells survive shear forces in the body and invade the immune system. Recent development in my period of research is the use of microfluidic devices and high-throughput techniques to understudy in a larger scale, cellular deformability and the viscoelastic properties of cells, and also in the identification of novel biomarkers such as the regulation of cytoskeletal proteins and extracellular matrix proteins for early cancer diagnosis.Current experimental challenges involved in this field are the complexity of the tumor microenvironment, scaling throughput, clinical translation, standardization and reproducibility, and integration of multimodal data. So, some of the significant findings I've established in this field from my past research and from my current research is that cancer cells can be distinguished from normal cells based on their responses to mechanical stressors. And the extent to which resist mechanical stressors is dependent on structural integrity, which is dictated to a reasonable extent by the presence of cytoskeletal proteins like the filamentous actin and other structural proteins within the cell.Our protocol offers a simple, repeatable, and non-destructive approach to characterize cultured cells mechanically. This is in contrast to other techniques like AFM and optical tweezers, which require a certain level of technical expertise in order to perform an experiment in a relatively low throughput. Within the use of the shear acid technique, will greatly advance the single cell mechanical characterization, and will also aid in many different cross-sectional areas such as rare disease diagnosis, personalized diagnosis and care, and monitoring single-cell drug interactions.

Watch this Scientific Journal Video about Microfluidic Shear Apparatus Setup for Single-Cell Fluid Shear Assay at JoVE.com

Microfluidic Shear Apparatus Setup for Single-Cell Fluid Shear Assay  

Begin the protocol by preparing the shear assay viscous flow media. To do so, preheat the base culture media for about 10 to 20 minutes at 60 to 70 degrees Celsius using a magnetic stir or hot plate. While continuously stirring the media, gently add methylcellulose into it to help the methylcellulose particles disperse quickly without coagulating.Allow this process to continue for approximately 15 to 24 hours to ensure a clear homogenous mix of media and cellulose. To set up the shear apparatus, attach a rubber gasket to the flow path to fasten the connection between the flow chamber and the Petri dish, and ensure a controlled uniform flow of single cells. The rubber gasket comes in different sizes depending on the desired flow profile and area of observation.Next, set up the shear assay system comprising of 60 or 100 milliliter dual syringes connected to a programmable syringe pump for infusion and withdrawal of the viscous culture media. Fill a syringe with the prepared viscous flow media and attach the filled syringe to its respective location on the syringe pump. Then, attach an empty syringe to the other syringe location on the syringe pump.Using 1/16th inch tubing and tubing connectors, connect both syringes to the flow chamber. Program the pump to infuse and withdraw a certain volume of fluid at a designated rate, and select the corresponding syringes. An example program is shown on the screen.Aspirate the cell culture media from the Petri dish to prepare for shear. Next, insert and fix the flow chamber with the rubber gasket onto the Petri dish containing the attached cells. Place the fitted microfluidic flow chamber with the cells in the dish onto an inverted microscope connected to a display monitor.The setup is now ready for applying fluid shear stress onto a single cell and optically monitoring the resulting cellular deformation.

microfluidic-shear-apparatus-setup-for-single-cell-fluid-shear-assay

Research

JoVE Journal

Cancer Research

145 次观看.  University of Notre Dame. 通过制备剪切测定粘性流动介质开始协议。为此，使用磁力搅拌或热板将基础培养基在60至70摄氏度下预热约10至20分钟。在不断搅拌介质的同时，轻轻地将甲基纤维素添加到其中，以帮助甲基纤维素颗粒快速分散而不会凝结。让此过程持续约15至24小时，以确保培养基和纤维素的清晰均匀混合物。要设置剪切装置，请将橡胶垫圈连接到流路以固定流动室和培养皿之间的连?...